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Metabolic engineering of Basfia succiniciproducens for the production of carbon-three compounds (Band 7)
Cód:
491_9783736999138
Metabolic engineering of Basfia succiniciproducens for the production of carbon-three compounds (Band 7)
Autor:
Editora:
Código:
491_9783736999138
Vendido e entregue por Um Livro
BeschreibungThe recent rise of bio-succinic acid as an industrial platform chemical has brought the rumen bacterium Basfia succiniciproducens, a natural producer of this organic acid, into the focus of research. This work investigated the potential of B. succiniciproducens for the production of carbon-three chemicals from sugars. Beside the optimization of improved genetic tools, the C3 chemicals alanine, ß-alanine and 3-hydroxypropionate (3-HP) were targeted. A new alanine producer, B. succiniciproducens ALA-1, exhibited good performance in the bioprocess. The next step demonstrated the proof-of-concept for ß-alanine synthesis using the heterologous expression of aspartate 1 decarboxylase genes from Corynebacterium glutamicum or Vibrio natriegens. The expression of two additional genes enabled the de novo synthesis of up to 100 mg L-1 3-HP from glucose.
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